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Submit a Sequence to PSORTb version 3.0.3

Based on a study last performed in 2010, PSORTb v3.0.2 is the most precise bacterial localization prediction tool available. PSORTb v3.0.2 has a number of improvements over PSORTb v2.0.4. Version 2 of PSORTb is maintained here.

PSORTb v3.0.3, released in February 2020, is the latest version. This mitigates a bug found in v3.0.2 when used to analyze archaeal cell envelopes when the view-results-via-email option was selected.

If you are planning a large volume of requests, including planning a course that could generate a large volume of requests from your course participants, please contact us beforehand. Thank you!

You can currently submit one or more Gram-positive or Gram-negative bacterial sequences or archaeal sequences in FASTA format (?). Copy and paste your FASTA-formatted sequences into the textbox below or select a file containing your sequences to upload from your computer. Web display mode is limited to the analysis of approximately 100 proteins. For larger analyses, either enter your email address in the form below (results of up to 5000 per submission returned by email) or for even larger analyses we can help you or you can download the standalone version.

See also:

Choose an organism type (?):
Choose Gram stain (?):
Advanced Gram stain options (?):
Output format (?):
Show results (?):
Email address:

Copy and paste your FASTA sequences below

or upload from file:
(uploads limited to 50KB, approximately 100 proteins, in Web display mode, enter an email address to use email mode if you need to analyze more proteins)

This is the most current version of the PSORTb program for bacterial protein subcellular localization prediction. PSORTb v3.0 now handles archaeal sequences as well as Gram-positive and Gram-negative bacterial sequences. Plus, it has several other improvements. Version 2 of PSORTb is maintained here (but we strongly recommend using version 3.0! ;-). If you are looking for a eukaryotic localization predictor, please visit to access other resources.  
PSORTb is maintained by the Brinkman Laboratory, Simon Fraser University, British Columbia, Canada. If you use PSORTb in your research, we would greatly appreciate if you cited one of the following publications, and note the version of software you use:
  • PSORTb v3.0: N.Y. Yu, J.R. Wagner, M.R. Laird, G. Melli, S. Rey, R. Lo, P. Dao, S.C. Sahinalp, M. Ester, L.J. Foster, F.S.L. Brinkman (2010) PSORTb 3.0: Improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes, Bioinformatics 26(13):1608-1615

  • PSORTb v2.0: J.L. Gardy, M.R. Laird, F. Chen, S. Rey, C.J. Walsh, M. Ester, and F.S.L. Brinkman (2005) PSORTb v.2.0: expanded prediction of bacterial protein subcellular localization and insights gained from comparative proteome analysis, Bioinformatics 21(5):617-623
  • PSORTb v1.0: Jennifer L. Gardy, Cory Spencer, Ke Wang, Martin Ester, Gabor E. Tusnady, Istvan Simon, Sujun Hua, Katalin deFays, Christophe Lambert, Kenta Nakai and Fiona S.L. Brinkman (2003) PSORT-B: improving protein subcellular localization prediction for Gram-negative bacteria, Nucleic Acids Research 31(13):3613-17

If you are interested in being notified about changes in the status of PSORTb, please subscribe to the psort-update mailing list by emailing with "subscribe psort-update" in the subject or body of the message.


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